hpac cells atcc atcc atcc  (ATCC)

Journal: Antioxidants

Article Title: Nimbolide Inhibits SOD2 to Control Pancreatic Ductal Adenocarcinoma Growth and Metastasis

doi: 10.3390/antiox12101791

Figure Lengend Snippet: SOD2 overexpression is associated with poor survival in pancreas adenocarcinoma: ( A ) heat-map representation of SOD1, SOD2, SOD3, and CAT expression in different cancer tissue including pancreatic cancer; ( B ) immunohistochemistry analysis of SOD2 expression in normal pancreas and different stages of human pancreatic ductal adenocarcinoma tumor tissues; SOD2-positive cells indicated by brown staining at 40× magnification (NT—normal tissue, MT—malignant tissue, MTS1—malignant tissue stage 1; MTS2—malignant tissue stage 2, MTS3—malignant tissue stage 3, MTS4—malignant tissue stage 4); ( C ) expression of SOD2 in pancreatic adenocarcinoma based on tumor grade using UALCAN; ( D ) SOD2 expression in normal, tumor, and metastatic pancreatic adenocarcinoma cells were compared using TNMplot analysis; ( E ) Western blot and ( F ) densitometric analysis of SOD2 expression in a panel of pancreatic cancer cell lines and normal pancreas cell line; Each bar represents the mean ± SEM of three separate experiments, * p < 0.05; ( G ) immunofluorescence analysis of SOD2 expression in pancreatic ductal adenocarcinoma cells compared with normal pancreas cells at 100× magnification via confocal microscopy; ( H ) Kaplan–Meier plot for overall survival; ( I ) recurrence-free survival (months) for SOD2 expression.

Article Snippet: Pancreatic duct normal epithelial cell line (hTERT-HPNE) and pancreatic ductal adenocarcinoma cell lines such as HPAC, AsPC-1, Capan-1, Capan-2, PANC-1, MIAPaCa-2, and BxPC-3 were procured from the American Type Culture Collection (ATCC-CRL-2119, Manassas, VA, USA) and maintained at 37 °C in a humidified carbon dioxide incubator set to 5%.

Techniques: Over Expression, Expressing, Immunohistochemistry, Staining, Western Blot, Immunofluorescence, Confocal Microscopy